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Isolation and quantitation of M‐factor, a diffusible mating factor from the fission yeast Schizosaccharomyces pombe
Author(s) -
Davey John
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070406
Subject(s) - schizosaccharomyces pombe , biology , mating type , yeast , mating , schizosaccharomyces , fission , mating of yeast , genetics , microbiology and biotechnology , saccharomyces cerevisiae , gene , physics , quantum mechanics , neutron
Although there have been several reports demonstrating the existence of mating factors in the fission yeast Schizosaccharomyces pombe it has not been possible to isolate these factors as cell‐free preparations. Such an ability is the first requirement towards a molecular characterization of these factors and here I report the successful isolation of a mating factor from S. pombe . This factor, termed M‐factor, is released by cells of the cellular mating type M (Minus) and induces mating‐specific changes in P‐type cells. A reliable and accurate assay for the quantitation of the M‐factor, based upon changes in cell volume following exposure to the factor, is also described.