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Inositol biosynthesis: Candida albicans and Saccharomyces cerevisiae genes share common regulation
Author(s) -
Klig Lisa S.,
Antonsson Bruno,
Schmid Elisabeth,
Friedli Laurence
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070403
Subject(s) - biology , candida albicans , microbiology and biotechnology , southern blot , saccharomyces cerevisiae , mutant , complementation , gene , inositol , corpus albicans , plasmid , northern blot , rna , biochemistry , genetics , receptor
The Candida albicans inositol biosynthetic gene and its regulation have been studied. The gene, CalNO1 , was cloned on a multicopy vector by complementation of a Saccharomyces cerevisae mutant strain. Southern blot analysis established that the cloned DNA was C. albicans genomic DNA in origin; neither rearrangements nor pseudogenes were evident. Blot hybridization analysis using RNA isolated from C. albicans revealed that a single RNA species (1·8 kilobases) was homologous to the cloned DNA fragment. The steady‐state levels of these transcripts were shown to be regulated in response to inositol in the growth media. In addition, the steady‐state levels of the RNA encoded by the cloned C. albicans DNA present in S. cerevisiae on a plasmid (YRpCalNO1) were regulated in response to exogenously provided inositol. The cloned C. albicans DNA fragment was shown to restore inositol‐1‐phosphate synthase activity to a S. cerevisiae mutant strain defective in this enzyme. This activity was also shown to be regulated in response to the presence of inositol in the growth media.