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III . Yeast sequencing reports. Determination of the sequence of the yeast YCL313 gene localized on chromosome III. Homology with the protein disulfide isomerase (PDI gene product) of other organisms
Author(s) -
Scherens B.,
Dubois E.,
Messenguy F.
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070212
Subject(s) - protein disulfide isomerase , biology , gene , gene product , akt1s1 , microbiology and biotechnology , yeast , nucleic acid sequence , endoplasmic reticulum , schizosaccharomyces pombe , homology (biology) , peptide sequence , biochemistry , hspa2 , hspa9 , genetics , saccharomyces cerevisiae , gene expression
We have determined the nucleotide sequence of the YCL313 gene as part of the YIp5 A1G clone localized on the left arm of chromosome III. This YCL313 gene encodes a protein of 522 amino acids (MW 58·3 kDa) which has large homologies with the human, mouse, chicken, bovine and rat PDI gene products. In these organisms the PDI gene encodes the protein disulfide isomerase (EC 5.3.4.1) also called S–S rearrangase, an enzyme that catalyses the rearrangements of S–S bonds in proteins. This enzyme is probably involved in protein folding within the lumen of the endoplasmic reticulum. These sequence homologies suggest that YCL313 is the yeast equivalent of the PDI gene. Gene disruption of YCL313 leads to a lethal phenotype indicating that this gene is essential for cell survival.