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Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica
Author(s) -
Fournier Ph.,
Guyaneux L.,
Chasles M.,
Gaillardin C.
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070104
Subject(s) - yarrowia , biology , extrachromosomal dna , plasmid , yeast , mutant , autonomously replicating sequence , genetics , escherichia coli , saccharomyces cerevisiae , genome , microbiology and biotechnology , dna , gene , origin of replication
Previous attempts to isolate autonomously replicating sequences ( ars ) from the dimorphic yeast Yarrowia lipolytica have been unsuccessful. We isolated a Fil − mutant unable to produce hyphae and growing only in a yeast form to facilitate ars isolation. This mutant was transformed with a Y. lipolytica DNA bank and several unstable clones were obtained. Extrachromosomal plasmids were evidenced in yeast recovered in Escherichia coli and characterized by restriction mapping. They were able to retransform Fil − and Fil + yeast strains at high frequency and transformants displayed a slightly unstable phenotype. The detailed analysis of the plasmids showed that only two different ars sequences had been isolated, each of them corresponding to a unique sequence in the Y. lipolytica genome. We concluded that functional ars sequences that can be cloned on plasmids are rare in this yeast.