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The complete sequence of the 8·2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase
Author(s) -
Thierry Agnès,
Fairhead Cécile,
Dujon Bernard
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060609
Subject(s) - biology , orfs , genetics , ura3 , stop codon , gene , open reading frame , chromosome , sequence (biology) , start codon , inverted repeat , reading frame , plasmid , microbiology and biotechnology , genome , peptide sequence , base sequence
We report here the DNA sequence of a segment of chromosome III extending over 8·2 kb. The sequence was determined using the random clone strategy followed by oligonucleotide‐directed sequencing. The segments contains five long open reading frames, YCR521, 522, 523, 524 and 526 , with only short distances between them. YCR523 (333 codons) endodes a ribokinase, a new function for yeast. YCR526 originates inside the MAT cassette, which is in continuity with the present segment, and extends over 358 codons outside of MAT . YCR524 (923 codons) codes for a putative membrane protein. YCR521, 522 and 524 , have each been disrupted by insertion of a URA3 cassette and are non‐essential genes. An active ARS element is located within YCR523 or its vicinity.