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Divergence and conservation of SUP2(SUP35) gene of yeasts Pichia pinus and Saccharomyces cerevisiae
Author(s) -
Kushnirov Vitaly V.,
TerAvanesyan Michael D.,
Didichenko Svetlana A.,
Smirnov Vladimir N.,
Chernoff Yuri O.,
Derkach Irina L.,
Novikova Olga N.,
IngeVechtomov Sergey G.,
Neistat Michael A.,
Tolstorukov Ilya I.
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060603
Subject(s) - biology , saccharomyces cerevisiae , divergence (linguistics) , yeast , gene , botany , genetics , linguistics , philosophy
SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF‐1α‐like protein factor, intimately involved in the control of translational accuracy in yeast Saccharomyces cerevisiae . In the present study a SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of the temperature‐sensitive sup2 mutation of S. cerevisiae . The nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82·4 kDa, exceeding the Sup2 protein of S. cerevisiae by 6 kDa. Like the SUP2 gene product of S. cerevisiae , the Sup2 protein of P. pinus represents a fusion of a unique N‐terminal part of a region homologous to EF‐1α. The comparison of amino acid sequences of the Sup2 proteins reveals high conservations (76%) of the C‐terminal region and low conservation (36%) of the N‐terminal part where, in addition, the homologous correspondence is ambiguous. Proteins related to the Sup2 of S. cerevisiae where found in P. pinus and some other yeast species by the immunoblotting technique. The relation between the evolutionary conservation of different regions of the Sup2 protein and their functional significance is discussed.