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Mapping of the trifunctional fatty acid synthetase gene FAS2 on chromosome XVI of Saccharomyces cerevisiae
Author(s) -
Siebenlist Ursula,
Nix Johanna,
Schweizer Michael,
Jäger Daniel,
Schweizer Eckhart
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060506
Subject(s) - centromere , biology , saccharomyces cerevisiae , centimorgan , tetrad , yeast , chromosome , gene , meiosis , mutant , microbiology and biotechnology , genetics , gene mapping , biochemistry
The trifunctional FAS2 gene encoding subunits α of the Saccharomyces cerevisiae fatty acid synthetase complex was mapped on the left arm of chromosome XVI 24 centinorgans proximal to GAL4 and 39 centimorgans distal and PEP4 relative to the centromere. Mapping was achieved by three‐independent methods: meiotic co‐segragation of FAS2 and ARO7 in recombination‐deficient spo11 ‐mutants; tetrad analysis of crosses between FAS2, GAL4 and PEP4; and Southern hybridization of purified FAS2 DBA with individual yeast chromosomes separated by pulsed‐field gel electrophoresis.