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Analysis of the THR4 region on chromosome III of the yeast Saccharomyces cerevisiae
Author(s) -
Mannhaupt Gertrud,
Van Der Linden Gerard,
Vetter Irene,
Maurer Kick,
Pilz Ursula,
Planta Rudi,
Feldmann Horst
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060408
Subject(s) - biology , open reading frame , saccharomyces cerevisiae , gene , genetics , complementation , terminator (solar) , homology (biology) , amino acid , mutant , biochemistry , peptide sequence , ionosphere , physics , astronomy
The gene encoding theonine synthase ( THR4 ) from the yeast Saccharomyces cerevisiae was cloned by complementation of a thr4 mutant. This gene was also found on a lambda clone (5239) consisting of a fragment of chromosome III inserted in the vector lambda MG3. The THR4 gene encodes a protein of 514 amino acids (M.W. 58 kDa), which has extensive homologies with E. coli threonine synthase ( thr C) and B. subtilis threonine synthase. The 5′ flanking region of the gene contains three regulatory sequences. [TGACT(C)] for the general amino acid control (GCN). About 130 bp downstream of the THR4 gene another open reading frame (563 amino acids) is found in the opposite orientation. This may imply that this open reading frame, called CTR86 , shares a terminator region with THR4 . The function of the protein encoded by CTR86 is not yet clear, but the fact that the upstream region contains a GCN4 responsive site that the gene product may also be involved in amino acid biosynthesis.