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Gaba transport in Saccharomyces cerevisiae
Author(s) -
McKelvey Joyce,
Rai Rajendra,
Cooper Terrance G.
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060311
Subject(s) - extracellular , biology , vacuole , biochemistry , efflux , intracellular , saccharomyces cerevisiae , azide , biophysics , proton transport , arsenate , yeast , chemistry , membrane , arsenic , organic chemistry , cytoplasm
Abstract Gamma‐aminobutyrate (GABA) accumulation in growing cultures of Saccharomyces cerevisiae was shown to occur by means of an active transport system that is inhibited by proton ionophores, azide, fluoride and arsenate ions. Transport occurred maximally at pH 5·0 and exhibited apparent K m values of 12 μ M and 0·1 m M . Accumulated GABA did not efflux upon treatment with proton ionophores and exchanged with extracellular material only very slowly. However, release was complete upon treatment with nystatin. These observations raise the possibility that a major portion of intracellular GABA is sequestered in the vacuole. The response of GABA uptake to growth on various nitrogen sources suggested that uptake may be subject to several types of regulation.