Premium
A K 2 neutral Saccharomyces cerevisiae strain contains a variant K 2 M genome
Author(s) -
Wingfield Brenda D.,
Southgate Valerie J.,
Pretorius Isak S.,
Van Vuuren Hendrik J. J.
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060210
Subject(s) - heteroduplex , biology , saccharomyces cerevisiae , rna silencing , homology (biology) , genome , yeast , strain (injury) , rna , genetics , microbiology and biotechnology , dna , gene , rna interference , anatomy
K 2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K 2 toxin. The M dsRNA was larger than the K 2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K 2 neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K 2 M genome had homology with K 2 neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K 2 M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K 2 neutral yeast strain has been characterized.