A K 2  neutral  Saccharomyces cerevisiae  strain contains a variant K 2  M genome | Zendy

Wingfield Brenda D. | Zendy; Southgate Valerie J. | Zendy; Pretorius Isak S. | Zendy; Van Vuuren Hendrik J. J. | Zendy

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A K 2 neutral Saccharomyces cerevisiae strain contains a variant K 2 M genome

Author(s) -

Wingfield Brenda D.,

Southgate Valerie J.,

Pretorius Isak S.,

Van Vuuren Hendrik J. J.

Publication year - 1990

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.320060210

Subject(s) - heteroduplex , biology , saccharomyces cerevisiae , rna silencing , homology (biology) , genome , yeast , strain (injury) , rna , genetics , microbiology and biotechnology , dna , gene , rna interference , anatomy

K 2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K 2 toxin. The M dsRNA was larger than the K 2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K 2 neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K 2 M genome had homology with K 2 neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K 2 M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K 2 neutral yeast strain has been characterized.

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