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Cloning and sequencing of the ornithine carbamoyltransferase gene from Pachysolen tannophilus
Author(s) -
Skrzypek M.,
Borsuk P.,
Maleszka R.
Publication year - 1990
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320060208
Subject(s) - biology , ornithine carbamoyltransferase , complementation , gene , microbiology and biotechnology , saccharomyces cerevisiae , genetics , biochemistry , mutant , amino acid , ornithine , arginine
A fragment of DNA from a yeast Pachysolen tannophilus , bearing the ornithine carbamoyltransferase gene (OCTase, EC 2.1.3.3) has been cloned from a genomic library by functional complementation of the Escherichia coli OCT‐negative mutant. The gene was located within the cloned segment of DNA and its coding sequence identified by DNA sequencing. This has indicated that P. tannophilus OCT gene encodes a 347 amino acid polypeptide, which shows 60% identity to the homologous Saccharomyces cerevisiae protein. The amino acid composition of its N‐terminus indicates that this protein is translocated across the mitochondrial membrane. The gene can be expressed in E. coli as well as in S. cerevisiae. Comparison with other OCTases confirms a high degree of conservation among these proteins.