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Induction of a heat shock‐type response in fission yeast following nitrogen starvation
Author(s) -
Walker Graeme M.,
McWilliams Philip G.
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050607
Subject(s) - schizosaccharomyces pombe , yeast , biology , heat shock protein , fission , starvation , shock (circulatory) , nitrogen , mutant , heat shock , microbiology and biotechnology , cell division , cell , schizosaccharomyces , saccharomyces cerevisiae , biochemistry , chemistry , gene , medicine , physics , organic chemistry , quantum mechanics , endocrinology , neutron
When cells of the fission yeast, Schizosaccharomyces pombe , are incubated in medium devoid of a nitrogen source, they accelerate into cell division and differentially synthesize two polypeptides at 46 and 27 kD (named p46 and p27) after a delay of about an hour. The synthesis of p46 and p27 is transient. These proteins have no obvious cell cycle connection since they are also evident in nitrogen‐starved (but not accelerated) cells of the temperature‐sensitive mutant of S. pombe , wee 1·50h − . We infer from this that p46 and p27 are synthesized as a direct result of nutritional stress. The possibility that p46 and p27 represent examples of general environmental stress proteins was investigated by comparing nitrogen starvation with the heat‐shock response in S. pombe. Heat‐shock analysis of cells revealed the existence of two proteins of similar Mr to p46 and p27. In addition, nitrogen‐starved cells acquired thermotolerance in a manner similar to heat‐shocking cells. We suggest that nitrogen starvation in fission yeast induces a subset of the total array of heat‐shock proteins.