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Cloning and sequencing of the gene for apocytochrome b of the yeast Kluyveromyces lactis strains WM27 (NRRL Y‐17066) and WM37 (NRRL Y‐1140)
Author(s) -
Brunner Aurora,
Coria Roberto
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050310
Subject(s) - kluyveromyces lactis , biology , schizosaccharomyces pombe , gene , open reading frame , genetics , homology (biology) , kluyveromyces , coding region , genome , intron , saccharomyces cerevisiae , yeast , peptide sequence
The apocytochrome b genes from two strains of the yeast Kluyveromyces lactis , have been isolated and sequenced. The coding sequences in strains WM27 (NRRL Y‐17066) and WM37 (NRRL Y‐1140) were identical but the upstream noncoding regions were slightly different. The sequences demonstrated the presence of a continuous open reading frame with no introns. The amino acid sequence, derived from the coding strand, showed 82% homology to the apocytochrome b of Saccharomyces cerevisiae strain D273–10B and only 58% homology to the protein from Schizosaccharomyces pombe strain 50. CUN and CGN codon families were absent from the K. lactis gene. Codon usage was very similar to that of other mitochondrial genomes with mostly U or A in the third position. There were two unusual features. All threonines were coded by ACA(U) and all arginines by AGA.