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Kinetics of growth and glucose transport in glucose‐limited chemostat cultures of Saccharomyces cerevisiae CBS 8066
Author(s) -
Postma Erik,
Alexander Scheffers W.,
Van Dijken Johannes P.
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050305
Subject(s) - chemostat , dilution , industrial fermentation , biology , kinetics , biochemistry , fermentation , saccharomyces cerevisiae , chromatography , chemistry , yeast , bacteria , thermodynamics , physics , genetics , quantum mechanics
The glucose transport capacity of Saccharomyces cerevisiae CBS 8066 was studied in aerobic glucose‐limited chemostat cultures. Two different transport systems were encountered with affinity constants of 1 and 20 m M , respectively. The capacity of these carriers ( V max ) was dependent on the dilution rate and the residual glucose concentration in the culture. From the residual glucose concentration in the fermenter and the kinetic constants of glucose transport, their in situ contribution to glucose consumption was determined. The sum of these calculated in situ transport rates correlated well with the observed rate of glucose consumption of the culture. The growth kinetics of S. cerevisiae CBS 8066 in glucose‐limited cultures were rather perculiar. At low dilution rates, at which glucose was completely respired, the glucose concentration in the fermenter was constant at 110 μ M , independent of the glucose concentration in the reservoir. At high dilution rates, characterized by the occurrence of both respiration and alcoholic fermentation, the residual substrate concentration followed Monod kinetics. In this case, however, the overall affinity constant was dependent on the reservoir glucose concentration.