Premium
Structure and nuclear localization signal of the SK13 antiviral protein of Saccharomyces cerevisiae
Author(s) -
Rhee SangKi,
Icho Tateo,
Wickner Reed B.
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050304
Subject(s) - biology , nuclear localization sequence , gene , rna , nuclear protein , saccharomyces cerevisiae , cell nucleus , nuclear export signal , gene product , fusion protein , microbiology and biotechnology , genetics , gene expression , recombinant dna , transcription factor
The yeast chromosomal genes SK12 , SK13 , SK14 , SK16 , SK17 and SK18 repress the replication of double‐stranded RNA viruses, protecting the host from the otherwise lethal effects of the virus. We cloned and sequenced the SK13 gene and found that it encodes a 163 kDa protein including a typical nuclear localization signal. Cell fractionation experiments show that the SK13 gene product is indeed tightly associated with nuclei and that the putative nuclear localization sequence directs β‐galactosidase into the nucleus. However, fusion of a part of the SK13 protein lacking this signal with β‐galactosidase directs β‐galactosidase into the nucleus, suggesting the presence of a second nuclear localization signal. The SK13 gene is only essential in the presence of an M double‐stranded RNA virus.