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Vanadate inhibition of mitochondrial respiration and H + ATPase activity in Saccharomyces cerevisiae
Author(s) -
Henderson Gillian E.,
Evans Ivor H.,
Bruce Ian J.
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050109
Subject(s) - vanadate , oligomycin , biology , atpase , saccharomyces cerevisiae , submitochondrial particle , mitochondrion , biochemistry , respiration , enzyme , yeast , botany
The effects of vandate on mitochondrial respiration and H + ATPase activity in Saccharomyces cerevisiae were studied. A 50% inhibition of oxygen uptake in isolated mitochondria was produced by 4·4 m M ‐V 2 O 5 . Activity of H + ATPase in whole mitochondria was inhibited by 50% by 5·5 μ M ‐V 2 O 5 , in submitochondrial particles by 55 μ M ‐V 2 O 5 ; and in the chloroform‐released H + ATPase by 0·5 m M ‐V 2 O 5 . Vandate was also found to relieve growth inhibition caused by the mitochondrial H + ATPase inhibitors NN′‐decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H + ATPase in S. cerevisiae .