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Extraction and rapid inactivation of proteins from Saccharomyces cerevisiae by trichloroacetic acid precipitation
Author(s) -
Wright Anthony P. H.,
Bruns Michael,
Hartley Brian S.
Publication year - 1989
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320050107
Subject(s) - trichloroacetic acid , biology , saccharomyces cerevisiae , yeast , polyacrylamide gel electrophoresis , extraction (chemistry) , biochemistry , gel electrophoresis , chromatography , precipitation , sodium , enzyme , chemistry , physics , organic chemistry , meteorology
Methods currently used for the extraction of proteins from yeast involve relatively long time periods between sampling cells from a culture and analysis of their proteins by polyacrylamide gel electrophoresis–sodium dodecylsulphate. Often it is desirable to inactivate cellular metabolism rapidly after sampling and here we show that trichloroacetic acid precipitation techniques, often used for rapid extraction and inactivation of proteins from higher eukaryotes, can be adapted for use with organisms which have cell walls.