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The influence of oxygen and organic hydrogen acceotors on glycolytic dioxide production in Brettanomyces anomalus
Author(s) -
Gaunt Davis M.,
Degn Hans,
Lloyd David
Publication year - 1988
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320040403
Subject(s) - acetaldehyde , aeration , oxygen , anaerobic exercise , fermentation , hydrogen production , biochemistry , biology , glycolysis , food science , chemistry , metabolism , ethanol , organic chemistry , catalysis , ecology , physiology
The yeast Brettanomyces anomalus showed the Custers effect in that under strictly anaerobic conditions, in the presence of glucose, CO 2 production was negligble. CO 2 production was stimulated by mixing anaerobic cell suspensions with an aerated glucose solution in astopped‐flow cell. Glycolytic CO 2 production continued even after oxygen exhaustion. Studies using an open reaction vessel showed that the rate of glycolytic CO 2 production could be increased to a maximum level by exposing the anaerobic cell suspension to brief pulses of O 2 . A cell suspension CO 2 at a maximal rate demonstrated the Pasteur effect on switching the mobile gas to a mixture conatining oxygen (5.05 KPa). In contrast to glycolytic CO 2 production in vivo nicotinamide pool responded rapidly to changes in oxygen concentration. The addition of acetaldehyde, acetone, or 3‐hydroxy‐butan‐2‐one led to a temprorary production of CO 2 at an initial rate depending on the concentration of substance added according to the Michaelis–Menten equation. The maximal rates were equal with all three substances, whereas tha apparent K m values were different. The total amount of CO 2 produced was 22‐fold greater than the amount of acetaldehyde added. Added organic hydrogen acceptors modulated the intracellular reedox balance of B. anomalus under conditions. These results are discussed in relation to the current hypothesis of the Custers effect.