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Karyotyping of yeast strains of several genera by field inversion gel electrophoresis
Author(s) -
Johnston John R.,
Contopoulou C. Rebecca,
Mortimer Robert K.
Publication year - 1988
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320040304
Subject(s) - biology , karyotype , yeast , saccharomyces cerevisiae , pulsed field gel electrophoresis , gel electrophoresis , chromosome , genetics , microbiology and biotechnology , electrophoresis , genotype , gene
Field inversion gel electrophoresis as been used to improve the resolution of the large chromosomes (>1000 kb) present in Saccharomyces kluyveri and in several genera of yeasts other than Saccharomyces cerevisiae , and thus establish more accurately the electrophoretic karyotype of these yeasts. Field inversion gel electrophoresis has also been used to demonstrate the presence of chromosome length polymorphisms in serveral of the yeasts studied. By Southern blotting techniques the greater degree of relatedness of S. Kluyveri and Kluyberomyces lactis to S. cerevisiae , as compared to that of the other genera of yeasts studied, has been established.