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Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism
Author(s) -
Lewis T. L.,
Keesler G. A.,
Fenner G. P.,
Parks L. W.
Publication year - 1988
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320040203
Subject(s) - sterol , ergosterol , biology , saccharomyces cerevisiae , mutant , biochemistry , sterol o acyltransferase , wild type , mutagenesis , yeast , cholesterol , gene , lipoprotein
Sterol uptake control mutants ( upc − ) have been isolated via ethylmethanesulfonate mutagenesis from wild‐type Saccharomyces cerevisiae . These mutants are heme and sterol competent but possess the ability to accumulate exogenous sterol(s) under aerobic conditions. Previous demonstrate sterol uptake only in a hem − , erg − background; however, the Upc − strains described here are Hem + and do not require exogenous sterol for growth. We were unable to obtain viable hem + , erg − , upc + recombinants; such combinations appear to be lethal. Isolates of Upc mutants demonstrated different levels of sterol uptake, and sterol analysis revealed a broad phenotypic range with regard to amounts and accumulation of ergosterol and non‐ergosterol sterols. Assays of acyl CoA: ergosterol acyltransferase and sterol ester hydrolase showed no apparent difference in activity between Upc mutants and the wild type.