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Changes in robosomal properties during adenylate deprivation in the cells of Kluyveromyces lactis
Author(s) -
Ishiguro Junpei,
Azuma Yukimasa,
Uritani Masahiro,
Miyasaki Masazumi
Publication year - 1988
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320040107
Subject(s) - biology , ribosome , adenylate kinase , kluyveromyces lactis , ribosomal rna , ribosomal protein , mutant , biochemistry , protein biosynthesis , rna , microbiology and biotechnology , yeast , enzyme , saccharomyces cerevisiae , gene
In an adenine‐requiring mutant strain of the yeast, Kluyveromyces lactis the intracellular content of ATP is one‐third to one‐fifth that in a protophic wild strain under growing conditions. The quantitatives difference becomes rather small in resisting stationary‐phase cells. Temporary changes in the two‐dimensional protein patterns of mutant ribosomes occur when the ATP content during the transition phase of growth. The transfer of exponentially growing cells to a synthetic complete medium void of adenine induces the same changes in mutant ribosomes within several hours. Identification of robosomal proteins by two‐dimensional gel electrophoresis indicated all changeable proteins (at least five proteins) to belong to 40S ribosomal subunits. The mutant ribosomes prepared from the transitio‐phase cells have much lower activity (below 60%) for poly(U)‐directed polyphenylalanine synthesis than those in exponentially growing or resisting stationary‐phase cells. Thus, changes in ribosomal components associated with the differences in ribosomes activity in a cell‐free system were noted in the adenylate‐deprived cells of K. lactix .