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Analysis of DNA sequences homologous with the ARS core consensus in Saccharomyces cerevisiae
Author(s) -
Bouton Amy H.,
Stirling Vivianne B.,
Smith M. Mitchell
Publication year - 1987
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320030207
Subject(s) - biology , genetics , consensus sequence , gene , homology (biology) , plasmid , locus (genetics) , histone h4 , restriction map , histone , microbiology and biotechnology , peptide sequence
We have previously identified an autonomously replicating segment ( ARS ) near the 3′ end of the histone H4 gene at the copy‐I H3‐H4 locus. We have now searched for additional autonomously replicating segments and sequences homologous with the ARS core consensus sequence near the copy‐II histone H4 gene and both of the histone H3 genes. No new ARS elements were identified by functional cloning assays. However, several matches to the ARS core consensus element were found within the DNA sequencs of the copy‐I and copy‐II genes. An exact match to the ARS core consensus was identified in the region downstream from the copy‐I histone H3 gene and a set of sequences with weak homology was also locatd within the copy‐II region. However, restriction fragments including these sequences did not demonstrate ARS activity on a plasmid in transformed cells.