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Identification of polypeptides of the carbon metabolism machinery on the two‐dimensional protein map of Saccharomyces cerevisiae . Location of 23 additional polypeptides
Author(s) -
Bataillé Nelly,
Peypouquet MarieFrance,
Boucherie Hélian
Publication year - 1987
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320030104
Subject(s) - saccharomyces cerevisiae , biology , biochemistry , yeast , enzyme , polyacrylamide gel electrophoresis , metabolism , gel electrophoresis , overproduction
Using a modification of the basic two‐dimensional polyacrylamide gel electrophoresis technique, we have undertaken a systematic identification of the polypeptides of the protein map of Saccharomyces cerevisiae corresponding to components of the carbon metabolism machinery. To the previous location of nine glycolytic enzyme polypeptides on the yeast protein map we add the location of 23 polypeptides. Ten of them were identified as corresponding to cytoplasmic enzymes of the carbon metabolism machinery and 13 were characterized as mitochondrial proteins. The criteria used to establish the identification of these polypeptides spots include migration with purified proteins, immunodetection, overproduction by plasmid–carrying strains and physiological behaviour.