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Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains
Author(s) -
Chen JunSong,
Beckley Janel R.,
Ren Liping,
Feoktistova Anna,
Jensen Michael A.,
Rhind Nicholas,
Gould Kathleen L.
Publication year - 2016
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3172
Subject(s) - schizosaccharomyces pombe , biology , genetics , cytokinesis , gene , schizosaccharomyces , mitosis , chromosome , cell division , chromosome segregation , saccharomyces cerevisiae , cell
The fission yeast model system Schizosaccharomyces pombe is used to study fundamental biological processes. To continue to fill gaps in the Sz. pombe gene deletion collection, we constructed a set of 90 haploid gene deletion strains covering many previously uncharacterized genes. To begin to understand the function of these genes, we exposed this collection of strains to a battery of stress conditions. Using this information in combination with microscopy, proteomics and mini‐chromosome loss assays, we identified genes involved in cell wall integrity, cytokinesis, chromosome segregation and DNA metabolism. This subset of non‐essential gene deletions will add to the toolkits available for the study of biological processes in Sz. pombe . Copyright © 2016 John Wiley & Sons, Ltd.