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Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis
Author(s) -
Ikeda Atsuko,
Kajiwara Kentaro,
Iwamoto Kunihiko,
Makino Asami,
Kobayashi Toshihide,
Mizuta Keiko,
Funato Kouichi
Publication year - 2016
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3138
Subject(s) - complementation , biology , saccharomyces cerevisiae , biosynthesis , yeast , mutant , phenotype , function (biology) , genetics , gene , biochemistry
ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae . Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1 Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans. Copyright © 2015 John Wiley & Sons, Ltd.