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New mechanisms that regulate Saccharomyces cerevisiae short peptide transporter achieve balanced intracellular amino acid concentrations
Author(s) -
Melnykov Artem V.
Publication year - 2016
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3137
Subject(s) - biology , saccharomyces cerevisiae , intracellular , transporter , amino acid , biochemistry , peptide , microbiology and biotechnology , yeast , gene
Abstract The budding yeast Saccharomyces cerevisiae is able to take up large quantities of amino acids in the form of di‐ and tripeptides via a short peptide transporter, Ptr2p. It is known that PTR2 can be induced by certain peptides and amino acids, and the mechanisms governing this upregulation are understood at the molecular level. We describe two new opposing mechanisms of regulation that emphasize potential toxicity of amino acids: the first is upregulation of PTR2 in a population of cells, caused by amino acid secretion that accompanies peptide uptake; the second is loss of Ptr2p activity, due to transporter internalization following peptide uptake. Our findings emphasize the importance of proper amino acid balance in the cell and extend understanding of peptide import regulation in yeast. Copyright © 2015 John Wiley & Sons, Ltd.