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A vector system for efficient and economical switching of a ura4 + module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe
Author(s) -
Chen Yinghui,
Chen Lihua,
An Ke,
Wang Yamei,
Jin Quanwen
Publication year - 2015
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3088
Subject(s) - biology , schizosaccharomyces pombe , plasmid , vector (molecular biology) , schizosaccharomyces , set (abstract data type) , antibiotics , microbiology and biotechnology , genetics , computer science , yeast , recombinant dna , dna , programming language , gene , saccharomyces cerevisiae
We describe here the development of a set of plasmid vectors that allow simple, efficient and economical switching of a ura4 + module in existing Schizosaccharomyces pombe strains to any of the three routinely used antibiotic marker cassettes, kanMX6 , hphMX6 and natMX6 . In principle, the applications of this system can also be extended to switching ura4 + for additional MX6 module‐based cassettes, such as bleMX6 , as long as the antibiotic marker has been cloned into an ura4 + module‐switching vector. We illustrate the application of this set of vectors in exchange of the ura4 + marker in existing strains with three antibiotic marker cassettes with high efficiency. Copyright © 2015 John Wiley & Sons, Ltd.