A vector system for efficient and economical switching of a  ura4 +   module to three commonly used antibiotic marker cassettes in  Schizosaccharomyces pombe | Zendy

Chen Yinghui | Zendy; Chen Lihua | Zendy; An Ke | Zendy; Wang Yamei | Zendy; Jin Quanwen | Zendy

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A vector system for efficient and economical switching of a ura4 + module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe

Author(s) -

Chen Yinghui,

Chen Lihua,

An Ke,

Wang Yamei,

Jin Quanwen

Publication year - 2015

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.3088

Subject(s) - biology , schizosaccharomyces pombe , plasmid , vector (molecular biology) , schizosaccharomyces , set (abstract data type) , antibiotics , microbiology and biotechnology , genetics , computer science , yeast , recombinant dna , dna , programming language , gene , saccharomyces cerevisiae

We describe here the development of a set of plasmid vectors that allow simple, efficient and economical switching of a ura4 + module in existing Schizosaccharomyces pombe strains to any of the three routinely used antibiotic marker cassettes, kanMX6 , hphMX6 and natMX6 . In principle, the applications of this system can also be extended to switching ura4 + for additional MX6 module‐based cassettes, such as bleMX6 , as long as the antibiotic marker has been cloned into an ura4 + module‐switching vector. We illustrate the application of this set of vectors in exchange of the ura4 + marker in existing strains with three antibiotic marker cassettes with high efficiency. Copyright © 2015 John Wiley & Sons, Ltd.

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