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Novel transporters from Kluyveromyces marxianus and Pichia guilliermondii expressed in Saccharomyces cerevisiae enable growth on l ‐arabinose and d ‐xylose
Author(s) -
Knoshaug Eric P.,
Vidgren Virve,
Magalhães Frederico,
Jarvis Eric E.,
Franden Mary Ann,
Zhang Min,
Singh Arjun
Publication year - 2015
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3084
Subject(s) - xylose , arabinose , biochemistry , saccharomyces cerevisiae , glucose transporter , pentose , biology , galactose , yeast , transporter , kluyveromyces marxianus , fermentation , gene , insulin , endocrinology
Genes encoding l ‐arabinose transporters in Kluyveromyces marxianus and Pichia guilliermondii were identified by functional complementation of Saccharomyces cerevisiae whose growth on l ‐arabinose was dependent on a functioning l ‐arabinose transporter, or by screening a differential display library, respectively. These transporters also transport d ‐xylose and were designated KmAXT1 (arabinose–xylose transporter) and PgAXT1 , respectively. Transport assays using l ‐arabinose showed that KmAxt1p has K m 263 m m and V max 57 n m /mg/min, and PgAxt1p has K m 0.13 m m and V max 18 n m /mg/min. Glucose, galactose and xylose significantly inhibit l ‐arabinose transport by both transporters. Transport assays using d ‐xylose showed that KmAxt1p has K m 27 m m and V max 3.8 n m /mg/min, and PgAxt1p has K m 65 m m and V max 8.7 n m /mg/min. Neither transporter is capable of recovering growth on glucose or galactose in a S. cerevisiae strain deleted for hexose and galactose transporters. Transport kinetics of S. cerevisiae Gal2p showed K m 371 m m and V max 341 n m /mg/min for l ‐arabinose, and K m 25 m m and V max 76 n m /mg/min for galactose. Due to the ability of Gal2p and these two newly characterized transporters to transport both l ‐arabinose and d ‐xylose, one scenario for the complete usage of biomass‐derived pentose sugars would require only the low‐affinity, high‐throughput transporter Gal2p and one additional high‐affinity general pentose transporter, rather than dedicated d ‐xylose or l ‐arabinose transporters. Additionally, alignment of these transporters with other characterized pentose transporters provides potential targets for substrate recognition engineering. Accession Nos: KmAXT1 : GZ791039; PgAXT1 : GZ791040 Copyright © 2015 John Wiley & Sons, Ltd.