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Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium: it enables the molecular investigation of dimorphism
Author(s) -
Papp László,
Sipiczki Matthias,
Holb Imre J.,
Miklós Ida
Publication year - 2014
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3048
Subject(s) - schizosaccharomyces pombe , biology , mycelium , fetal bovine serum , inducer , hypha , ploidy , yeast , bovine serum albumin , genome , liquid medium , biochemistry , genetics , botany , gene , in vitro , saccharomyces cerevisiae , chromatography , chemistry
The non‐pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus , could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has a haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which would enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was fetal bovine serum (FBS). Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein‐containing compounds, such as peptone and bovine serum albumin or amino acids, proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sz. japonicus could be improved by lower external pH and higher temperature. Copyright © 2014 John Wiley & Sons, Ltd.