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Purification of G 1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure
Author(s) -
Marbouty Martial,
Ermont Caroline,
Dujon Bernard,
Richard GuyFranck,
Koszul Romain
Publication year - 2014
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3005
Subject(s) - elutriation , biology , saccharomyces cerevisiae , yeast , population , enumeration , genome , computational biology , genetics , gene , mathematics , chemistry , demography , organic chemistry , combinatorics , sociology
Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G 1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell‐cycle biases when conducting genome‐wide/single‐cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S‐phase progression of four yeast species ( S. cerevisiae , Candida glabrata , Lachancea kluyveri and Pichia sorbitophila ) and could theoretically be applied to any culture of single, individual cells. Copyright © 2014 John Wiley & Sons, Ltd.