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Poster Sessions
Author(s) -
Beck, Sabrina,
Montilla Martinez, Malayko,
Kr\ufcger, Vivien,
Meinecke, Michael,
Schliebs, Wolfgang,
Wagner, Richard,
Erdmann, Ralf
Publication year - 2013
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2973
Subject(s) - citation , library science , biology , information retrieval , computer science , world wide web
growth modulation of protein kinase/phosphatase mutants DTU Orbit (31/12/2018) Time dependent physiological characterization of yeast oxidative stress response and growth modulation of protein kinase/phosphatase mutants The objective of the project was to investigate the time-dependent batch growth effects of oxidative environmental conditions on protein ki nase (PK) and phosphatase (PP) deletion mutants and relevant wild type strains of Saccharomyces cerevisiae . To achieve this goal, 44 different PK and PP mutants were selected for their known activities in various stress response pathways, including oxidative stress, and were investigated for their response to oxidative stress. Hydrogen peroxide was us ed as the oxidizing agent at a number of different concentrations ranging from mild to moderate stress (0.25, 0.50 and 1.0 mM). Understanding the growth physiology of S. cerevisiae allows us to estimate the link between genotype and stress-response phenotype. Growth physiology parameters, such as growth rate, diauxic shift times, stress-induced stasis times, were measured in fermentative batch cultures as important in dicators of cellular fitness. We used a high-throughput microfermentation system (BioLector, m2p-labs) to retrieve these indicators of cellular fitness and perform our batch fermentations.