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Yeast on the milky way: genetics, physiology and biotechnology of Kluyveromyces lactis
Author(s) -
Rodicio Rosaura,
Heinisch Jürgen J.
Publication year - 2013
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2954
Subject(s) - kluyveromyces lactis , biology , tetrad , eukaryote , kluyveromyces , saccharomyces cerevisiae , yeast , genetics , model organism , crispr , computational biology , human genetics , saccharomyces , microbiology and biotechnology , genome , gene
The milk yeast Kluyveromyces lactis has a life cycle similar to that of Saccharomyces cerevisiae and can be employed as a model eukaryote using classical genetics, such as the combination of desired traits, by crossing and tetrad analysis. Likewise, a growing set of vectors, marker cassettes and tags for fluorescence microscopy are available for manipulation by genetic engineering and investigating its basic cell biology. We here summarize these applications, as well as the current knowledge regarding its central metabolism, glucose and extracellular stress signalling pathways. A short overview on the biotechnological potential of K. lactis concludes this review. Copyright © 2013 John Wiley & Sons, Ltd.