z-logo
Premium
The influence of nitrate on the physiology of the yeast Dekkera bruxellensis grown under oxygen limitation
Author(s) -
Barros Pita Will,
Tiukova Ievgeniia,
Leite Fernanda Cristina Bezerra,
Passoth Volkmar,
Simões Diogo Ardaillon,
Morais Marcos Antonio
Publication year - 2013
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2945
Subject(s) - yeast , nitrate , biology , fermentation , ammonium , biochemistry , pentose phosphate pathway , oxygen , nitrogen assimilation , sugar , nitrogen cycle , food science , metabolism , botany , nitrogen , glycolysis , ecology , chemistry , organic chemistry
A previous study showed that the use of nitrate by Dekkera bruxellensis might be an advantageous trait when ammonium is limited in sugarcane substrate for ethanol fermentation. The aim of the present work was to evaluate the influence of nitrate on the yeast physiology during cell growth in different carbon sources under oxygen limitation. If nitrate was the sole source of nitrogen, D. bruxellensis cells presented slower growth, diminished sugar consumption and growth‐associated ethanol production, when compared to ammonium. These results were corroborated by the increased expression of genes involved in the pentose phosphate (PP) pathway, the tricarboxylic acid (TCA) cycle and ATP synthesis. The presence of ammonium in the mixed medium restored most parameters to the standard conditions. This work may open up a line of investigation to establish the connection between nitrate assimilation and energetic metabolism in D. bruxellensis and their influence on its fermentative capacity in oxygen‐limited or oxygen‐depleted conditions. Copyright © 2013 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here