Premium
D‐Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae
Author(s) -
Stewart Benjamin J.,
Navid Ali,
Kulp Kristen S.,
Knaack Jennifer L. S.,
Bench Graham
Publication year - 2013
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2942
Subject(s) - methylglyoxal , biochemistry , biology , metabolism , catabolism , saccharomyces cerevisiae , lactoylglutathione lyase , carbohydrate metabolism , glycolysis , dihydroxyacetone , yeast , metabolite , enzyme , glycerol
Methylglyoxal, a reactive, toxic dicarbonyl, is generated by the spontaneous degradation of glycolytic intermediates. Methylglyoxal can form covalent adducts with cellular macromolecules, potentially disrupting cellular function. We performed experiments using the model organism Saccharomyces cerevisiae , grown in media containing low, moderate and high glucose concentrations, to determine the relationship between glucose consumption and methylglyoxal metabolism. Normal growth experiments and glutathione depletion experiments showed that metabolism of methylglyoxal by log‐phase yeast cultured aerobically occurred primarily through the glyoxalase pathway. Growth in high‐glucose media resulted in increased generation of the methylglyoxal metabolite d ‐lactate and overall lower efficiency of glucose utilization as measured by growth rates. Cells grown in high‐glucose media maintained higher glucose uptake flux than cells grown in moderate‐glucose or low‐glucose media. Computational modelling showed that increased glucose consumption may impair catabolism of triose phosphates as a result of an altered NAD + :NADH ratio. Copyright © 2013 John Wiley & Sons, Ltd.