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Characterization of glycolytic metabolism and ion transport of Candida albicans
Author(s) -
Calahorra Martha,
Sánchez Norma Silvia,
Peña Antonio
Publication year - 2012
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2915
Subject(s) - fermentation , respiration , candida albicans , oxidative phosphorylation , biology , biochemistry , bioenergetics , alternative oxidase , atpase , metabolism , glycolysis , saccharomyces cerevisiae , corpus albicans , yeast , enzyme , mitochondrion , microbiology and biotechnology , botany
Abstract The main energetic pathways, fermentation and respiration, and the general ion transport properties of Candida albicans were studied. Compared to Saccharomyces cerevisiae , we found that in C. albicans : (a) the cell mass yield when grown in YPD was significantly larger; (b) it required longer times to be starved of endogenous substrates; (c) ethanol production was lower but significant; (d) respiration was also lower; (e) it showed a small activity of an alternative oxidase; (f) fermentation and oxidative phosphorylation seemed to compete for both ADP and NADH; and (g) NADH levels were lower. Regarding ion transport and compared to S. cerevisiae : (a) the general mechanism was similar, with a plasma membrane H + ‐ATPase that generates both a plasma membrane ΔpH and a ΔΨ, the latter being responsible for driving K + inside; (b) its acidification capacity is slightly smaller and less sensitive to activation by high pH; and (c) the presence of K + results in a large activation of both respiration and fermentation, most probably due to the energy required in the process. ADP produced by H + ‐ATPase stimulation by high pH or the addition of K + at low pH results in the increase of both respiration and fermentation. Copyright © 2012 John Wiley & Sons, Ltd.