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Molecular characterization of Candida boidinii MIG1 and its role in the regulation of methanol‐inducible gene expression
Author(s) -
Zhai Zhenyu,
Yurimoto Hiroya,
Sakai Yasuyoshi
Publication year - 2012
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2909
Subject(s) - biology , gene , methanol , gene expression , cytosol , heterologous expression , biochemistry , saccharomyces cerevisiae , microbiology and biotechnology , regulation of gene expression , recombinant dna , enzyme , chemistry , organic chemistry
Methanol‐inducible gene promoters in methanol‐utilizing yeasts are used in high‐level heterologous gene expression systems. Generally, expression of methanol‐inducible genes is completely repressed by the presence of glucose. In this study we identified the MIG1 gene in Candida boidinii , which encodes a homologue of the glucose repressor Mig1p of Saccharomyces cerevisiae . Disruption of the CbMIG1 gene had no growth effect on various carbon sources. Activation of the methanol‐inducible AOD1 gene, which encodes alcohol oxidase, was increased in the early stage of methanol induction when cells of the CbMIG1 ‐disrupted strain were transferred from glucose medium to methanol medium. Furthermore, CbMig1p tagged with yellow fluorescent protein was primarily localized in the nucleus of glucose‐grown cells, but was diffuse in the cytosol of methanol‐grown cells. This cytosolic diffusion in methanol‐grown cells occurred in a CbMsn5p‐dependent manner. These results suggest that CbMig1p is involved in negative regulation of methanol‐inducible gene expression in C. boidinii . Copyright © 2012 John Wiley & Sons, Ltd.