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Construction of an insertion marker collection of Sz. japonicus (IMACS) for genetic mapping and a fosmid library covering its genome
Author(s) -
Furuya Kanji,
Aoki Keita,
Niki Hironori
Publication year - 2012
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.2907
Subject(s) - fosmid , biology , genetics , schizosaccharomyces pombe , genome , schizosaccharomyces , genetic marker , identification (biology) , genomic library , gene mapping , chromosome , contig , computational biology , gene , saccharomyces cerevisiae , base sequence , botany
Measuring relative genetic distances is one of the best ways to locate genetic loci. Here we report the construction of a strains set for genetic mapping in Schizosaccharomyces japonicus , which belongs to the genus Schizosaccharomyces together with the well‐studied fission yeast Sz. pombe . We constructed 29 strains that bear a positive‐negative selection marker at different loci. The marker was inserted every 500 kb in the genome of Sz. japonicus . Each marker thus becomes a ‘scale mark’ of a chromosome that behaves like a yardstick. By determining the genetic distances from the inserted markers, the relative location of a genomic mutation can be determined. We also constructed a fosmid library that covers an entire genome of Sz. japonicus . These tools together would facilitate identification and cloning of the gene. Copyright © 2012 John Wiley & Sons, Ltd.