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An improved method for whole protein extraction from yeast Saccharomyces cerevisiae
Author(s) -
Zhang Tingting,
Lei Jie,
Yang Hanjiang,
Xu Kun,
Wang Rui,
Zhang Zhiying
Publication year - 2011
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1905
Subject(s) - saccharomyces cerevisiae , yeast , biology , extraction (chemistry) , saccharomyces , protein purification , biochemistry , chromatography , computational biology , chemistry
Abstract A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS‐PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies. Copyright © 2011 John Wiley & Sons, Ltd.