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Functional characterization of the PP2C phosphatase CaPtc2p in the human fungal pathogen Candida albicans
Author(s) -
Feng Jinrong,
Zhao Jingwen,
Li Jing,
Zhang Lilin,
Jiang Linghuo
Publication year - 2010
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1778
Subject(s) - candida albicans , biology , corpus albicans , phosphatase , yeast , enzyme , gene , biochemistry , microbiology and biotechnology , saccharomyces cerevisiae , dna
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ion. There are seven PP2C‐like genes in Candida albicans . In this study, we demonstrate that CaPtc2p is a PP2C phosphatase. Surprisingly, in addition to the cytoplasmic localization, CaPtc2p is partially associated with mitochondria in yeast‐form and filamentous cells of C. albicans . Expression of CaPTC2 is developmentally regulated during the serum‐induced filamentation. Deletion of CaPTC2 renders C. albicans cells sensitive to SDS and azole antifungals, as well as the DNA methylation agent methylmethane sulphonate and the DNA synthesis inhibitor hydroxyurea. Therefore, CaPtc2p might fulfil multiple functions, including the regulation of mitochondrial physiology and checkpoint recovery from DNA damage in C. albicans cells. Copyright © 2010 John Wiley & Sons, Ltd.