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Identification and characterization of the type 2C protein phosphatase Ptc4p in the human fungal pathogen Candida albicans
Author(s) -
Zhao Jingwen,
Sun Xin,
Fang Jiaping,
Liu Wei,
Feng Chen,
Jiang Linghuo
Publication year - 2010
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1739
Subject(s) - biology , candida albicans , phosphatase , enzyme , biochemistry , gene , saccharomyces cerevisiae , fungal protein , corpus albicans , azole , microbiology and biotechnology , antifungal
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ions. There are seven PP2C genes, named from PTC1 to PTC7 , in Saccharomyces cerevisiae . In the current study we identified the CaPTC4 gene in Candida albicans and demonstrated that the CaPtc4p protein is a typical PP2C enzyme, which is highly conserved in fungal species. Deletion of CaPTC4 renders Candida cells sensitive to sodium and potassium ions as well as to antifungal azole drugs. In addition, we have shown that CaPtc4p is localized in the mitochondrion, suggesting that CaPtc4p is likely to be involved in the regulation of a mitochondrial function related to ion homeostasis. Copyright © 2009 John Wiley & Sons, Ltd.