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Isolation and characterization of Candida kefyr orotidine‐5′‐phosphate decarboxylase ( URA3 ) gene
Author(s) -
Tsang Paul WaiKei,
Wong KinSing,
Chu Jennifer KaMan
Publication year - 2010
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1732
Subject(s) - ura3 , biology , genbank , gene , genetics , kluyveromyces lactis , complementation , homology (biology) , open reading frame , mutant , saccharomyces cerevisiae , peptide sequence
Candida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr , the orotidine‐5′‐phosphate decarboxylase ( URA3 ) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura 3 auxotrophs of C. albicans and Saccharomyces cerevisiae . Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763. Copyright © 2009 John Wiley & Sons, Ltd.

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