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Sequence of the yeast protein expression plasmid pEG(KT)
Author(s) -
Pierce B. Daniel,
Wendland Beverly
Publication year - 2009
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1667
Subject(s) - plasmid , biology , fusion protein , saccharomyces cerevisiae , yeast , cloning (programming) , epitope , microbiology and biotechnology , sequence (biology) , expression vector , multiple cloning site , tandem affinity purification , protease , computational biology , recombinant dna , genetics , biochemistry , gene , antibody , enzyme , computer science , programming language , affinity chromatography
The plasmid pEG(KT) is a widely used plasmid for expressing high levels of GST fusion proteins in the yeast Saccharomyces cerevisiae . Unfortunately, a complete sequence file has been lacking, thus complicating efforts to design cloning projects or to modify the plasmid for other uses (e.g. exchanging selection markers, epitope tags or protease cleavage sites to remove the epitope tag). Here, the complete sequence of the pEG(KT) plasmid is reported, thus facilitating its use. Additionally, its use as a vector backbone for high‐level expression of a TAP‐tagged protein is shown. Copyright © 2009 John Wiley & Sons, Ltd.