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Repeated elements coordinate the spatial organization of the yeast genome
Author(s) -
O'Sullivan J. M.,
Sontam D. M.,
Grierson R.,
Jones B.
Publication year - 2009
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1657
Subject(s) - biology , genomic organization , genome , spatial organization , genetics , chromosome , computational biology , epigenomics , chromosome conformation capture , yeast , histone deacetylase , gene , histone , gene expression , evolutionary biology , enhancer , dna methylation
The spatial organization of the chromosomes is crucial for gene expression and development. Inter‐ and intrachromosomal interactions form a crucial part of this epigenomic regulatory system. Here we use circular chromosome conformation capture‐on‐chip (4C) to identify interactions between repetitive and non‐repetitive loci within the yeast genome. The interacting regions occur in non‐randomly distributed clusters. Furthermore, the SIR2 histone deacetylase has opposing roles in the organization of the inter‐ or intrachromosomal interactions. These data establish a dynamic domain model for yeast genome organization. Moreover, they point to the repeated elements playing a central role in the dynamic organization of genome architecture. Copyright © 2009 John Wiley & Sons, Ltd.