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Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays
Author(s) -
Minato Toshiko,
Yoshida Satoshi,
Ishiguro Tatsuji,
Shimada Emiko,
Mizutani Satoru,
Kobayashi Osamu,
Yoshimoto Hiroyuki
Publication year - 2009
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1654
Subject(s) - biology , gene , dna microarray , genetics , saccharomyces cerevisiae , genome , microarray , yeast , gene expression profiling , orthologous gene , gene expression
The bottom fermenting yeast Saccharomyces pastorianus is reported to have arisen as a natural hybrid of two yeast strains, S. cerevisiae and S. bayanus . The S. pastorianus genome includes S. cerevisiae ‐type (Sc‐type) genes and orthologous lager‐fermenting‐yeast specific‐type (Lg‐type) genes derived from S. cerevisiae and S. bayanus , respectively. To gain insights into the physiological properties of S. pastorianus , we developed an in situ synthesized 60‐mer oligonucleotide microarray for gene expression monitoring of these orthologous genes, consisting of approximately 6600 Sc‐type genes and 3200 Lg‐type genes. A comparison of the transcriptional profile of orthologous genes (e.g. Sc‐type and Lg‐type genes) in S. cerevisiae or S. bayanus demonstrated the feasibility of performing gene expression studies with this microarray. Genome‐wide analysis of S. pastorianus with this microarray could clearly distinguish more than 67% of the expressed orthologous genes. Furthermore, it was shown that the gene expression of particular Lg‐type genes differed from that of the orthologous Sc‐type genes, suggesting that some Lg‐type and Sc‐type genes may have different functional roles. We conclude that the oligonucleotide microarray that we constructed is a powerful tool for the monitoring of gene expression of the orthologous genes of S. pastorianus . Copyright © 2009 John Wiley & Sons, Ltd.