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A simple method for isolating disomic strains of Saccharomyces cerevisiae
Author(s) -
Zebrowski David C.,
Kaback David B.
Publication year - 2008
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1590
Subject(s) - biology , saccharomyces cerevisiae , simple (philosophy) , genetics , yeast , philosophy , epistemology
A simple method to select disomic (N + 1) strains that should be applicable for almost any chromosome in Saccharomyces cerevisiae is presented. A diploid heterozygous for a KanMX knock‐out mutation in an essential gene is sporulated and viable geneticin (G418)‐resistant colonies selected. Disomic products of a missegregation or non‐disjunction event containing a copy of both the wild‐type essential gene and its complementary KanMX knock‐out allele make up most of the viable colonies. This method has been used to isolate disomic haploids for a variety of chromosomes. It is appropriately named MARV (for m issegregation‐ a ssociated r estoration of v iability) and is easily adaptable to virtually any strain. Copyright © 2008 John Wiley & Sons, Ltd.