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Effects of high hydrostatic pressure on the growth and β‐carotene production of Rhodotorula glutinis
Author(s) -
Wang SuiLou,
Chen DeJing,
Deng BaiWan,
Wu XiaoZong
Publication year - 2008
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1583
Subject(s) - biology , rhodotorula , hydrostatic pressure , carotene , food science , biochemistry , thermodynamics , yeast , physics
The effects of high hydrostatic pressure (HHP) on the biomass and β‐carotene biosynthesis of Rhodotorula glutinis R68 were studied. After treatment with five repeated cycles at 300 MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/l, decreased by 8.5% compared to the parental strain R68, but its β‐carotene production reached 19.4 mg/l, increased by 52.8% compared to the parental strain R68. The result of restriction fragment length polymorphism (RFLP) analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced β‐carotene production in this strain as a result of gene mutation induced by HHP treatment. Copyright © 2008 John Wiley & Sons, Ltd.