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The spectrum of Trp − mutants isolated as 5‐fluoroanthranilate‐resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus
Author(s) -
Jones Elizabeth W.,
Berget Peter B.,
Burnette III James M.,
Anderson Candice,
AsafuAdjei Denise,
Avetisian Seda,
Barrie Fatmata,
Chen Ruby,
Chu Bur,
Conroy Samantha,
Conroy Sean,
Dill Allyson,
Eimer Will,
Garrity Diane,
Greenwood Alexander,
Hamilton Tamara,
Hucko Simon,
Jackson Carmen,
Livesey Kristen,
Monaco Tiffany,
Onorato Christina,
Otsuka Mai,
Pai Satyan,
Schaeffer George,
Shung Sharon,
Spath Samantha,
Stahlman Jonathan,
Sweeney Blake,
Wiltrout Elizabeth,
Yurovsky Daniel,
Zonneveld Andrea
Publication year - 2008
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1552
Subject(s) - biology , complementation , mutant , homothallism , heterokaryon , selection (genetic algorithm) , strain (injury) , ploidy , genetics , botany , gene , mating type , artificial intelligence , computer science , anatomy
Abstract 5‐Fluoroanthranilic acid (FAA)‐resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described. Copyright © 2007 John Wiley & Sons, Ltd.