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On‐line measurements of oscillating mitochondrial membrane potential in glucose‐fermenting Saccharomyces cerevisiae
Author(s) -
Andersen Ann Zahle,
Poulsen Allan K.,
Brasen Jens Christian,
Olsen Lars Folke
Publication year - 2007
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1508
Subject(s) - biology , membrane potential , glycolysis , depolarization , mitochondrion , biochemistry , inner mitochondrial membrane , anaerobic glycolysis , mitochondrial carrier , saccharomyces cerevisiae , nad+ kinase , nicotinamide adenine dinucleotide , chemiosmosis , biophysics , microbiology and biotechnology , yeast , metabolism , atp synthase , bacterial outer membrane , enzyme , escherichia coli , gene
We employed the fluorescent cyanine dye DiOC 2 (3) to measure membrane potential in semi‐anaerobic yeast cells under conditions where glycolysis was oscillating. Oscillations in glycolysis were studied by means of the naturally abundant nicotinamide adenine dinucleotide (NADH). We found that the mitochondrial membrane potential was oscillating, and that these oscillations displayed the same frequency and duration as the NADH oscillations. It was confirmed that DiOC 2 (3) localizes itself in the mitochondrial membrane and thus reports qualitative changes solely in mitochondrial membrane potential. Our studies showed that glycolytic oscillations perturb the mitochondrial membrane potential and that the mitochondria do not have any controlling effect on the dynamics of glycolysis under these conditions. Depolarization of the mitochondrial membrane by addition of FCCP quenched mitochondrial membrane potential oscillations and delocalized DiOC 2 (3), while glycolysis continued to oscillate unaffected. Copyright © 2007 John Wiley & Sons, Ltd.