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The ORF YNL274c ( GOR1 ) codes for glyoxylate reductase in Saccharomyces cerevisiae
Author(s) -
Rintala Eija,
Pitkänen JuhaPekka,
Vehkomäki MaijaLeena,
Penttilä Merja,
Ruohonen Laura
Publication year - 2007
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1434
Subject(s) - glyoxylate cycle , biochemistry , biology , saccharomyces cerevisiae , reductase , enzyme , glyoxysome , dehydrogenase , yeast
The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D ‐glycerate, using either NADPH or NADH as a co‐factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c ) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α‐ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase‐encoding gene leads to higher biomass concentration after diauxic shift. Copyright © 2006 John Wiley & Sons, Ltd.