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Substrate‐inducible versions of internal alternative NADH : ubiquinone oxidoreductase from Yarrowia lipolytica
Author(s) -
Garofano Aurelio,
Eschemann Andrea,
Brandt Ulrich,
Kerscher Stefan
Publication year - 2006
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.1426
Subject(s) - yarrowia , biology , biochemistry , alternative oxidase , neurospora crassa , respiratory chain , electron transport complex i , oxidoreductase , nad+ kinase , nadh dehydrogenase , dehydrogenase , ubiquinol , cofactor , yeast , saccharomyces cerevisiae , enzyme , oxidase test , gene , mitochondrion , coenzyme q – cytochrome c reductase , mutant , protein subunit , cytochrome c
In standard laboratory strains of the obligate aerobic yeast Yarrowia lipolytica , respiratory chain complex I (proton‐translocating NADH : ubiquinone oxidoreductase) is an essential enzyme, since alternative NADH dehydrogenase activity is located exclusively at the external face of the mitochondrial inner membrane. Deletions and other loss‐of‐function mutations in genes for nuclear coded subunits of complex I can be obtained only when an internal version of the latter enzyme, termed NDH2i, is introduced. In contrast to recent findings with Neurospora crassa , external alternative NADH dehydrogenase activity is dispensable in complex I deletion strains of Y. lipolytica . We used regulable promoters to create strains which express internal alternative NADH dehydrogenase in a substrate‐dependent manner. The ability to switch between complex I‐dependent and ‐independent mode of growth simply by changing the carbon source is an important prerequisite for screens for both loss‐of‐function and inhibitor resistance mutation. The isocitrate lyase promoter (pICL1), in combination with a NDH2i allele that results in reduced expression and activity, was most promising. In the presence of complex I inhibitors, this construct allowed growth on acetate, but not on glucose minimal media. A somewhat higher background was observed with the acyl‐CoA oxidase 2 (pPOX2) promoter on glucose minimal media. Copyright © 2006 John Wiley & Sons, Ltd.